FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11451343
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC168000.015.13897694-95
GTCTATG42100.014.600593
TATACCG41650.014.3794557
GTATAAG44350.012.9858153
TAGACGG15400.012.9632957
GCCGTAT234500.012.90416794-95
CGCCGTA272150.012.84138494-95
GTCTAGG36600.012.7094783
CGTATCA142450.012.64137994-95
CCTAGAC36500.012.224175
GTGTTAG22700.012.197583
GTGTATG55950.011.9760833
TATACTG52800.011.870467
TACACCG30400.011.7593917
GGTCGCC472100.011.67905494-95
GCCCTAG32550.011.5687763
TATCATT143800.011.55445394-95
GTATCAT140100.011.54489194-95
GTCTAGT33200.011.5090773
TCGCCGT353400.011.438704594-95
Produced by FastQC (version 0.11.5)