FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15810562
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC160050.016.57298794-95
GCCGTAT210850.014.53916494-95
CGTATCA126200.013.60763394-95
CGCCGTA249050.012.44887594-95
TGGTCGC457850.011.72923492-93
GGTCGCC476800.010.98027194-95
GTATAAG58250.010.3337353
TATACTG78550.010.107277
GTCGCCG573800.010.07449894-95
TCGCCGT381600.09.83966694-95
TCTCGGG85150.09.83858894-95
GGTGGTC562450.09.671690-91
CTACACT57150.09.66760356
GTGGTCG514150.09.63690592-93
GTATAGA65050.09.5930783
GTCTAGT45050.09.5615123
GTATCAT143350.09.42183594-95
GTCTAGG52500.09.361763
GTCCTAA51650.08.874313
TCGGTGG663700.08.85020388-89
Produced by FastQC (version 0.11.5)