FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9667172
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG15450.018.6313577
GTCTAGG29900.016.6324333
CCGTATC124450.014.48864694-95
CGTATCA112600.013.97310794-95
TAGAGCG36600.013.9538137
CCTAGAC33450.013.894265
TATACCG35350.013.3965227
GACGGTC19250.013.2644969
GTACCGT22550.012.9707858
ATAGACG19100.012.8832686
GTGTATG45500.012.7515333
GTATCAT110650.012.75128294-95
TACACCG25150.012.5531357
GGACTGC26300.012.5335738
CTAGACA47350.012.35475356
GTCTATG37000.012.2460663
CGCCGTA205100.012.0499694-95
GTCTAGT23950.011.9972663
GCCCTAG24350.011.5732593
AGGACAG28150.011.5451867
Produced by FastQC (version 0.11.5)