FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19175870
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG59850.014.816367
CCGTATC394600.014.69616594-95
CGCCGTA637550.013.98057294-95
GCCGTAT533850.013.91429794-95
TATCATT295850.013.73439394-95
ATCATTA256950.013.6642394-95
CGTATCA322400.013.40950394-95
GTCTAGG57500.013.1893963
GTATCAT303850.013.09784194-95
TCGCCGT843000.012.52778194-95
GTATAGG68850.012.3819473
CCTAGAC61900.012.3141855
TAGAGCG57200.012.174967
TCATTAA202250.012.1277894-95
TCTCGGG139600.012.10154594-95
GTCTATG70050.012.0908113
GTCGCCG1197400.011.9885294-95
GTATAGA71700.011.8897773
TGGTCGC1156250.011.87604792-93
CTAGACA86450.011.81502156
Produced by FastQC (version 0.11.5)