FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14999216
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGT3650.022.1759822-23
TCGACAG4750.019.88096224-25
TACCGGC3357.4578566E-1118.7925822-23
CGGTTAG4300.017.77968620-21
ACGACTA3652.564775E-1017.24798422-23
CACGATG6450.016.73259424-25
CCGTTAG2708.6618456E-716.65512724-25
GCGAGTA3102.0877451E-715.95637122-23
TTAGGCG4552.1827873E-1115.8132224-25
GTCGGTA2306.831531E-515.64133624-25
ACGTTCA4351.8553692E-1015.50649624-25
TCGATAG5250.015.4178924-25
TAGTCGT3901.14068825E-814.989614526-27
CGAATGC3405.94775E-714.54874424-25
GTATACA7900.014.4410991
AGTGCGC2501.3903744E-414.39002924-25
TACTGGC4705.9480953E-1014.35175726-27
ACAGCGC3154.3088494E-614.27582426-27
TAACGTG3154.30973E-614.27554122-23
CGTAATG4203.025525E-813.91892826-27
Produced by FastQC (version 0.11.5)