FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_DHA5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_DHA5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16199968
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT477000.014.80310394-95
CCGTCTT392500.014.607079594-95
TGCCGTC585550.014.57196494-95
CGTCTTC365950.013.65938594-95
GTCTTCT308300.012.705955594-95
ATGCCGT637950.012.69914694-95
TATGCCG711100.011.99585792-93
CGTATGC805350.010.5862290-91
TTCTGCT210000.010.5489894-95
GTATGCC778400.010.51906692-93
TATACCG52300.010.29591755
CTACACT45000.010.2427464
GTGTTAG37350.010.1402061
TCTTCTG272950.09.68748194-95
ACTGTGC54950.09.6333298
TCGTATG848900.09.62656990-91
CTCGTAT888800.09.543488-89
CTTCTGC236300.09.5197194-95
GTCTAGG42850.09.4776021
TAGACGG24000.09.316865
Produced by FastQC (version 0.11.5)