FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_DHA5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_DHA5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11293309
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13700.014.6593445
CCTAGAC34100.013.9214493
TGCCGTC293200.013.65165594-95
GCCGTCT240850.013.64380694-95
GCCCTAG29700.012.9088041
TATACCG41800.012.4483265
CCGTCTT206950.012.1737294-95
GTCTAGG35100.012.0931381
ATGCCGT315800.011.95929594-95
GGACTGC27650.011.8853536
GTCTTCT183200.011.82116994-95
CGTCTTC194900.011.59159194-95
ACCCTGG28150.011.5117971
TATACTG47800.011.4587115
GACAGGC35350.011.3626467
TAGAGCG41100.011.3276745
GACAGTG28450.011.0701387
GACTGTG16500.011.0652767
TATGCCG360900.011.05900192-93
GTCCTGG27750.011.0198321
Produced by FastQC (version 0.11.5)