FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12450633
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG17900.019.1307015
TATACCG41500.017.823365
TAGAGCG48800.017.589795
CCTAGAC41600.016.0253143
GTCTAGG43000.015.7141081
GTCTATG47850.014.8846681
CTAGACA61850.014.6172474
TAAGGTG62850.014.1661555
TAGAGGT49200.013.9208654
ATAGACG22200.013.7804024
GACTGTG20650.013.4878557
TTCTGCT183500.013.42274794-95
CTGTGCC56350.013.28860959
AGACACT37850.013.2693436
CTAGACT31400.013.2328024
GTACCGT33000.013.1441446
GCCGTCT397150.013.07049194-95
TGCCGTC482750.013.06042894-95
GTCCTGG30300.012.9584271
ACTGTCC33500.012.9484318
Produced by FastQC (version 0.11.5)