FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15001103
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG48150.015.354341
TAGACGG20300.014.3887765
GCCGTCT390500.013.80142394-95
TGCCGTC468150.013.60316194-95
GTACCGT33550.013.4672296
TATACCG54600.013.2905945
GTCTTCT283850.013.08671294-95
CGTCTTC300850.012.63542994-95
TAGAGCG55300.012.2970535
CCGTCTT336950.012.20265794-95
TAAGGCG26850.012.0685185
TCTTCTG260150.011.80555194-95
TTCTGCT182100.011.76577994-95
CTTCTGC218700.011.75818294-95
ATGCCGT513400.011.58644594-95
GTGTATG72450.011.4012541
CCTAGAC48950.011.375533
ACTGTCC39500.011.3234578
GTCTATG55350.011.2957561
CACTGAG82950.011.2243067
Produced by FastQC (version 0.11.5)