FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19045572
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG26200.017.4213455
TATACCG59750.016.7297365
TTCTGCT421450.015.61043694-95
GTCTAGG60050.015.50541
CCTAGAC60350.014.9005233
GTACCGT40500.014.763686
TCTGCTT368950.014.68968794-95
CTAGACA79650.014.3271184
TAGAGCG61550.014.2382335
CACTGAG85750.014.1591167
TATACTG87650.013.9561565
ACTGAGC85850.013.9300268
GTCTATG67350.013.8247861
CTGCTTG275200.013.7401294-95
TGCTTGA218400.013.5833694-95
GTGTATG90100.013.5761241
CTTCTGC502300.013.56572894-95
GCTTGAA174350.013.50747794-95
GCCGTCT1015750.013.04612394-95
GTCTTCT667950.012.96535194-95
Produced by FastQC (version 0.11.5)