FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13900151
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG19400.014.5948235
GCCGTCT314050.013.70391194-95
GTCTAGG42350.013.4785151
TGCCGTC380600.013.13733794-95
CCGTCTT272650.012.80365594-95
CGTCTTC248900.012.63109694-95
GTCTTCT231800.012.272694-95
TAGAGCG48500.012.0524985
CCTAGAC45100.011.7468543
ATGCCGT416900.011.6867794-95
TATACCG45750.011.6789465
GTATAAG62200.011.5998431
GTCTATG54400.011.1644721
TTCTGCT159800.011.15851194-95
GACGGTC22650.010.8877347
GTATAGA63450.010.7955571
TATGCCG469500.010.68374192-93
CTTCTGC185250.010.67312794-95
TCTTCTG212750.010.63496594-95
TATACTG59850.010.5298895
Produced by FastQC (version 0.11.5)