FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11451343
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG16400.019.4981615
GTCTAGG37150.016.1069771
ACTGTCC28050.015.3086328
GTCTATG40500.014.7746711
ACTGAGC55100.014.3428658
GCCGTCT370850.014.13327294-95
CCTAGAC36600.014.1043163
ATAGACG17200.014.0766784
TATACCG35700.013.9475455
TAGAGCG37400.013.924285
TGCCGTC447600.013.62321194-95
CCGTCTT312250.013.611382594-95
CACTGAG56950.013.31533057
TTCTGCT163650.013.24367494-95
GACTGCG17650.013.199677
CGTCTTC280150.013.149281594-95
CTAGACA51950.013.102484
GTCTTCT242950.012.80319994-95
GTATAGG46350.012.712811
GTACCGT26600.012.5365186
Produced by FastQC (version 0.11.5)