FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15810562
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC455550.014.08727294-95
GCCGTCT369450.014.03460794-95
CCGTCTT303850.012.70833794-95
GTCTTCT258100.012.68853594-95
CGTCTTC296100.012.570806594-95
ATGCCGT484400.011.94793194-95
TATGCCG533500.011.92193492-93
CGTATGC615700.010.3821290-91
TACACCG45300.010.3791545
GTCCTAG54050.09.7964191
GTATGCC595150.09.789680592-93
TTATACT87150.09.4809824
TATACTG77700.09.458625
TCTTCTG241050.09.29719594-95
GTCTAGA54700.09.2627661
GTACCGT38650.09.2121246
TTCTGCT184450.09.15589294-95
CTCGTAT699450.09.10961888-89
TACACTG58150.09.1060745
CCTAGAC52250.09.0864593
Produced by FastQC (version 0.11.5)