FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9667172
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13400.020.7879875
GTCTAGG31200.016.392121
CCTAGAC30300.015.9763513
GTACCGT22700.015.0875366
GTCTATG37550.015.0793771
TATACTG46100.014.7595385
CTAGACA44350.014.4159094
ACTGAGC48500.014.1235438
TAGAGCG37950.013.9583355
GCCGTCT265250.013.78936794-95
TACACCG22250.013.7509345
GACGGTC15650.013.7142857
TCTAGAC34750.013.4047823
TGCCGTC321150.013.30868494-95
CCGTCTT228300.013.22095394-95
TATACCG33150.013.0866195
CACTGAG51000.013.0729117
TCTATAC43500.012.9130923
AGTACCG28600.012.6138735
CTAAGAC25500.012.5363823
Produced by FastQC (version 0.11.5)