FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n01_CUI15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n01_CUI15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19175870
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG59600.015.3963445
GCCGTCT910800.014.99033794-95
TGCCGTC1098000.014.50704294-95
CCGTCTT737800.014.36309494-95
TTCTGCT352850.014.327538594-95
TATACCG55450.014.0786935
GTCTAGG60600.013.7854631
CCTAGAC57100.013.7525723
CTAGACA83650.013.6991174
TCTGCTT311800.013.45418594-95
CGTCTTC653500.013.42884794-95
GTCTTCT546050.012.86960794-95
GTCCTAA64800.012.6806171
GTATAGG68250.012.64161
GACTGTG39250.012.56176957
GTCTATG63400.012.4566071
TAGACGG28200.012.3036035
ATGCCGT1207100.012.254251594-95
GCTTGAA151600.012.1797294-95
CACTGAG91850.012.1276687
Produced by FastQC (version 0.11.5)