FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15879121
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCT5055.116097E-6321.654971
CTAACGT4050.0011351155267.383971
GGAACGT6251.1981871E-5259.897221
GGATGCG6501.401193E-5249.901171
CAGACTA4400.0014548654246.114781
CGTTAAA4600.0016619506235.414151
CTGAGCG4850.001947241223.27941
CGAATGT5100.0017856674212.334321
AACTCGA8253.626933E-5196.891831
GATAACG5700.0031572464189.983351
GGTAGGC5700.0031572464189.983351
GTAAGGC6250.003120252173.264821
GAGCTGA9556.499871E-5170.08981
GGAGCAT10057.9659076E-5161.627611
GGACCAG10158.286501E-5160.035221
CGCGTCT6900.005591271156.942761
GCGGCTA6950.0057133087155.813671
AGCCTAG10509.485059E-5154.700731
AATGCGT7050.0059626675153.603551
GGAGTCA7050.0059626675153.603551
Produced by FastQC (version 0.11.5)