FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_NON5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_NON5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12345013
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT26403.718469E-798.428831
GCGGATA38602.888186E-453.8553621
GACCACA29850.007077453752.2315861
GCGTATA20354.095504E-551.0765881
GGACACA41504.129694E-450.0919761
GCGGTTA32000.009316822548.722271
CGTTATG23102.4556357E-1047.1082732
GAGCCCA44305.698418E-446.9258961
GAGTCCT45456.4655824E-445.738551
TAGGCCG22850.045.4883421
CGATCTT54400.001565771538.213551
CGGATAG30101.3206954E-732.1358762
GGATCCA82353.124849E-431.55461
GCCTCAG30801.6158629E-731.4055182
CGTACAG23152.5882713E-531.3376852
GGAGTGT34103.6379788E-1230.481191
AGCCTTT68400.004811874630.3920611
TGGGCTA35251.8189894E-1229.4867651
CGGATTA28953.5386784E-629.235882
CGAGTAT26005.7153276E-527.9025922
Produced by FastQC (version 0.11.5)