FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_NON5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_NON5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9013645
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA23152.2190212E-590.39411
GAGCGTA26700.004430215358.781561
ACGTTAC18109.534415E-857.8072781
GGACATA30200.00721262351.9691241
TAGACCG20200.051.7976071
GGCCAGT21702.0099833E-848.2171331
GGCCTTC44605.692601E-446.9198041
GGCCATA47958.133435E-443.641781
CGTCCTA22851.2816599E-841.879032
GGGCCGT37600.041.7411581
CTGGCCT25500.041.0318341
CGCCTAA11750.001149102240.7206732
GGCTATT54550.001533781638.361571
TACAGCG13853.2544814E-437.7729871
TAGCGTT14255.313268E-936.712691
CAGGACA28600.036.5843241
CGCTATA14951.6591081E-434.9937021
CGTATAG21151.5006024E-533.9338952
CGTACTA24801.1496777E-633.7628562
CGTCTAG14200.002912211733.694922
Produced by FastQC (version 0.11.5)