FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9092867
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT12150.001449148987.309151
GGCCGTA20300.001415380278.384691
ACGTGCG15655.872858E-567.783141
GACTGTA15900.066.717371
CTGCCGT24300.00288871165.4818651
GCGTATA17201.5756745E-561.6747781
GCGTATT26700.004195254859.595851
CGCTCTA29050.005858210454.7748451
GGCCCCA30450.00705694252.2564621
GGGTCCG10206.022869E-452.00031
CGGATAG18452.1154847E-951.3373952
GAGGTCA41403.6841896E-451.2466741
CGCTATA14509.495125E-1048.991812
AAGTATA47357.1433355E-444.807021
CGTCCTA13304.1447805E-544.51012
TAGCGTG11952.5885075E-444.3851931
GGCCTTA36356.3760724E-443.774671
GCGTTTA25601.5474143E-841.4377441
GAGTGCG26100.040.643911
GGTCTAG18000.039.4656222
Produced by FastQC (version 0.11.5)