FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15001964
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTA35451.8557473E-458.908241
ACGTCTA28150.005514752655.638471
GACACTA28950.006161352654.1009671
GCGATCA31900.00904249249.0978931
GGATGTT53352.3612056E-548.9291761
GAGTGTG43000.048.5650521
GGACCTT56253.2285177E-546.4066051
GGCTGTA24950.001478854641.8496441
GACGGTC25600.040.7870561
CGCTATA24150.040.015232
GGGCTAG36700.039.4973222
GGACATA53200.001373215939.2537081
AAGTGTC27103.969035E-938.529471
AGTGTTA29102.3792381E-937.3595772
GAGGGTA71501.3282459E-436.5086941
GGACTTA43150.036.297171
CGGACAG17251.7056052E-435.0133252
GGTCTAG28300.034.147272
GGCTTTA46251.3333138E-433.8642771
CGTGGTA23152.6061656E-531.307812
Produced by FastQC (version 0.11.5)