FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11236970
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCCC21750.00195911272.232611
GCGTTGA30750.007720644651.091361
GAGCCGG45156.024127E-446.3952561
CAGTATA48258.3552516E-443.4144171
GACGTAC11650.001084606741.203932
CGCTATA18455.756623E-639.0264852
AGATATA69201.0740761E-437.8386151
CGTGTTA14350.0030184633.4512752
GCCTCTA38701.1914381E-931.0094182
TAGCGTT16951.6542072E-730.8959541
GAGTGTG34050.030.759851
CGGATTA27752.6970938E-630.2718962
GTACTCA35950.029.1341551
GTGACCA35953.6379788E-1229.1341551
CGTCTAG16750.006432630628.6582582
CGTATAA29604.458576E-628.3799042
GCCTCAG29704.577132E-628.2843482
GACGTGT19255.439506E-827.2044891
GTCATAA38952.7284841E-1126.890191
GCGTATA19501.1565223E-426.8557151
Produced by FastQC (version 0.11.5)