FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15851813
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA23359.677024E-10136.824521
AGCCTCA39003.2819244E-668.266081
GACATCA47056.808906E-445.2689061
CAGCATA50159.322563E-442.4706231
GGCCCCT53550.001287290739.7740751
GGCCTCT67950.039.1814161
GTACTTA28250.037.6973761
GCGTATA29251.7826096E-936.4085771
GTCCCTA20301.4085488E-534.2555352
CGGTCTA31908.745701E-932.6984632
AGTCTAG32259.727955E-932.3435972
GACCTCT50400.031.6949651
CGTACAA30051.8888386E-730.854682
CGCTATA26652.5065674E-930.442222
CCGTTAA17701.5021025E-430.0833571
GCGATCT71700.005385120429.7057441
CGTCTAA16050.00619266528.8842092
CATTCGC18857.3865467E-628.2480351
GAGGTTT95956.643128E-427.747551
AACCTGA38400.027.7330951
Produced by FastQC (version 0.11.5)