FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12659820
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT9550.00461709154.695141
GGCGTTA30550.007603622551.293481
GCGGGTA31700.00879935349.4326741
GGGCTGA37350.041.954911
GTCTACA25252.400453E-641.373361
GAGATCT68651.0413099E-438.04361
GGCATGT56300.001808066537.111091
AGCGGTT58450.002173263835.7460141
GAGTACA47400.033.0594061
CCCTATA49200.031.8499161
AAGAGCG49900.031.4031221
GAGTGTG33600.031.0915851
CGTATAG30001.8369974E-730.950342
CGTTCAG18853.629086E-430.7861482
TAACGTG17152.0765765E-730.4570641
CGTTCAA30652.2167296E-730.2939722
CGTATAA36403.1132004E-828.697092
GCCTCTA40752.9594958E-928.4819072
GACCTTA20455.847579E-428.377452
GGCTCTA74850.007291976427.9138891
Produced by FastQC (version 0.11.5)