FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10476102
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAC11500.00614303191.29391
CGTCCTA16500.00643819963.6290781
CGCTATA18801.9995059E-755.8446731
GAGTCCT43304.8421556E-448.4932981
GGAGCAT46857.140215E-444.818781
GGCCTAG16703.2222597E-642.464512
CGTTCAA28254.7293724E-1141.8381922
CGTGTTA14807.866652E-539.930032
AGAGGTA41450.037.993241
CGGACCC14157.3732845E-537.098231
AGCTCTA58300.002092434836.016461
GAGTGTG29303.45608E-1135.8320771
GGCTTCT59950.002399515635.0251851
CGAGTAG27858.146162E-833.951282
GCCTTAA21250.033.3721122
CGCGTTG15100.00417349431.3093782
CGTCCAA23203.0671134E-530.5671272
CGTACTA27202.5977643E-630.417292
CAGTCCT34900.030.0825181
GCCTCAG23703.54773E-529.9222532
Produced by FastQC (version 0.11.5)