FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649141
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA20800.001547794376.665291
ACGCATA23150.002366640468.882851
GCGGTTA28150.005135515756.647891
GGCTACA39252.8079154E-454.170291
GCGAATA53802.2306434E-549.4001851
GTGTTAT36050.044.2340661
GGAGGTA49809.081916E-442.6944581
CGCCTTA25650.002195230541.4460871
AGGTATA65657.225733E-540.4833221
ACGTTAA41200.038.7048071
CGTATCG15401.1087719E-437.6730882
CGGGCTA24903.582136E-837.2797132
GAGGGCA58550.002012489636.3139881
CCGTTAG15251.01468286E-434.8554761
CGTGGTA20301.39716485E-534.2954982
CGGATAG35456.693881E-1032.731482
CAGTGCT67950.031.290421
GTGTATA52050.030.6366581
CGCTATA23353.6377016E-529.8157862
GCGCTAT18253.8198777E-1029.1258091
Produced by FastQC (version 0.11.5)