FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n02_EXW15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n02_EXW15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24924122
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTT57250.090.3080751
GGCCCTA68653.468631E-852.718081
GTCCCTA40650.050.8746641
GCGCTAT30600.050.6876221
GCGCCTT61359.663854E-750.563691
CGCTATA33500.046.6036222
GCGGGTT83558.087494E-637.1284521
GGCTCCA71351.3923254E-436.2308121
GCGCCTA57200.00205965236.1548041
GGCTGTA44259.943008E-535.051781
CGTTTAG50900.033.0317342
GGAGGTA95752.0569736E-532.3977281
GCGGTTT65550.00401656831.5492741
GGACATA84853.851297E-430.4663371
GGTCTAG47850.030.1176072
CAGTATG68900.005125693530.0153121
GAGCCTA69100.005198851729.9284341
GGCTTCT122853.324436E-629.4594731
GCGAATA70800.00585461829.2098161
CGCCTTA46252.5465852E-1028.5628872
Produced by FastQC (version 0.11.5)