FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15879121
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT4950.018.39321324-25
CGTGGTA5900.017.21220426-27
ACGTTAG5950.017.06748222-23
TTTAACG6550.016.57329422-23
CATAGCG6150.016.51248424-25
ACGTAAC4250.016.47902926-27
TCTGCGA6700.016.20225122-23
AGACGTC6950.016.12336526-27
TTACGGT8950.016.04169724-25
CGCAGAG5500.015.91716822-23
CTAACGG4050.015.5634924-25
AGCGTAG5650.015.49458822-23
GTCGACA8600.015.47300324-25
TTAGCGC7700.015.46242724-25
AACGTGT4850.015.16233322-23
GCAGTCT7000.015.00765124-25
GCGACAA9150.014.92564224-25
ACGATTG8450.014.91888326-27
GCATTCG5200.014.81520922-23
GTCGGTA4500.014.78527922-23
Produced by FastQC (version 0.11.5)