FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_NON5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_NON5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12345013
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC496250.014.60359794-95
GCCGTCT407400.014.57432594-95
GTCTAGG34950.014.077691
CGTCTTC308550.013.97417594-95
GTCTTCT265800.013.94528894-95
CCGTCTT332450.013.76149394-95
TTCTGCT169900.012.94049794-95
GTATAGG61300.012.811291
ATGCCGT547200.012.75849694-95
TAGACGG18500.012.2350255
GACTGCG17850.011.9034257
TATGCCG610300.011.87816192-93
TATACCG41350.011.7298995
CTTCTGC191000.011.71649794-95
CCTAGAC37650.011.5337763
TAAGGTG53650.011.0209935
CTAGACA54550.010.9245524
TCTGCTT153050.010.90968894-95
TCTTCTG236850.010.79398294-95
CTATACT73650.010.6004094
Produced by FastQC (version 0.11.5)