FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_NON5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_NON5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9013645
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT238350.014.84687394-95
TGCCGTC294600.014.30156294-95
GTATAGG53150.013.979821
CCGTCTT199850.013.46521294-95
GTGTATG44250.012.6204231
GTCTTCT159950.012.59283994-95
CGTCTTC185750.012.53496494-95
ATGCCGT320550.012.48803794-95
TTCTGCT111400.012.44106294-95
GTCTAGG25600.012.3862551
TAGAGCG28950.012.2882835
CCTAGAC25050.011.9891523
CCAGGAC28000.011.8811173
GTGCTAG29350.011.7711781
TATACCG32700.011.7267975
TATGCCG359800.011.66478592-93
TAGACGG13300.011.4633465
GTGTAGG27000.011.3934371
GACCGTG20300.011.3788657
CTAGACA40300.011.00649454
Produced by FastQC (version 0.11.5)