FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9092867
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG14700.018.5445865
TATACCG33850.016.652665
GTCTAGG31550.015.4541711
CTAGACA43050.015.1341994
CCTAGAC32700.015.1199213
TAGAGCG35450.014.8583675
ACTGAGC50250.014.3436298
CACTGAG52350.014.2094527
GTCTAGA33450.014.01028251
GTCCTGG22250.013.8290131
GTGTATG46900.013.8278791
CGTCTTC189050.013.612494-95
GTCTATG38750.013.1934951
TAGGCAG25600.012.9949625
GCCGTCT236600.012.93681494-95
GTCTTCT171150.012.91698694-95
CCGTCTT207300.012.88204394-95
GACCGTG20000.012.7057647
ACTGTCC24050.012.67941958
CCTATGC32450.012.6732743
Produced by FastQC (version 0.11.5)