FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15001964
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG50950.016.8077471
TAGACGG20950.016.0981455
TATACCG47650.015.9977165
CCTAGAC50750.015.74988653
GCCGTCT582350.015.51975894-95
ACTGTCC36100.014.9726358
GTGTATG77750.014.7870311
GTACCGT35650.014.772236
TTCTGCT244300.014.63547194-95
CCGTCTT485100.014.61251194-95
TGCCGTC701350.014.51663594-95
ACTGAGC70750.014.300048
TAGAGCG52150.013.9971535
CTAGACA69700.013.9204694
CACTGAG75000.013.85905557
GTCTATG57150.013.4941791
CGTCTTC415850.013.46896694-95
AGTACCG42900.013.4613835
GTCTAGA55950.013.4453511
CTAAGAC45750.013.2296263
Produced by FastQC (version 0.11.5)