FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11236970
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT258600.015.92530694-95
TGCCGTC321300.015.32644794-95
CGTCTTC189750.014.57059794-95
CCGTCTT213450.014.53265294-95
GTCTTCT175900.013.94516694-95
TATACCG38750.013.47213655
ATGCCGT355450.013.1781594-95
TATGCCG395550.012.448915592-93
CCTAGAC34750.011.7000733
GTCTATG41600.011.2601881
TCTTCTG155450.010.99527394-95
GTATGCC440900.010.77032292-93
CGTATGC466850.010.60701390-91
TAGGGAG40200.010.4579145
TAGACGG16050.010.3623265
TTCTGCT116350.010.2633594-95
ACTGCGC27500.010.2468458
GTGTATG57000.010.2101951
CTTCTGC131150.010.12662194-95
GCCCTAG28050.010.1209711
Produced by FastQC (version 0.11.5)