FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15851813
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG19300.019.8732595
GACTGCG15150.016.1649067
GTCTAGG53450.015.3294471
GACAGTG36800.014.6908877
CCTAGAC49200.014.1837693
CTAGACA76400.013.9127934
ACCCTGG34900.013.7064211
TAGAGCG62850.013.6759025
TGCCGTC341950.013.62036594-95
TATACCG58200.013.5775525
GACGGTC19400.013.5763527
AGCGCGC36600.013.2562998
GCCGTCT278350.013.2299494-95
GTCTATG65700.013.0479211
CGTCTTC226000.012.46109194-95
GACAGGC46000.012.4558637
GGTGTCC53350.012.2989498
ACAGGCG45100.012.29472358
GAGCGCG44000.012.2869237
TAGAGGT70750.012.2803644
Produced by FastQC (version 0.11.5)