FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12659820
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG19300.018.4316925
TAGAGCG50500.017.0158395
GTCTATG50800.016.3147951
GTCTAGG41550.015.7295141
CCTAGAC42850.015.0951773
GCCGTCT288500.014.13871194-95
TATACCG47350.013.9523665
ACTGAGC66000.013.6487658
ATAGACG24150.013.5832044
GACGGTC21900.013.5000417
TGCCGTC350050.013.46061994-95
GTGGTAG68650.013.4524481
GTGTTAC20450.013.4320381
CTAGACA60650.013.4073484
AGTACCG37950.013.3909915
GTCCTGG30100.013.3739491
CCGTCTT248250.013.13742994-95
CACTGAG71800.012.9964917
GTCTAGA45800.012.7188211
GTACCGT29300.012.6128446
Produced by FastQC (version 0.11.5)