FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10476102
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG15600.019.5427865
GCCGTCT237050.015.7234594-95
TGCCGTC292850.015.30610194-95
CTAGACA47800.014.7863754
TATACCG39200.014.4939315
GTCTAGG38600.014.3464331
GACGGTC17150.014.2741367
CCGTCTT201700.013.91088194-95
CCTAGAC39150.013.8055053
CGTCTTC175650.013.69947294-95
ATGCCGT326650.013.36175294-95
TAGAGGT42150.013.2612974
TCTAGAC39500.013.0984273
GTCTATG38850.013.0358121
GGACTGC25650.012.7848196
GTGTTAC15700.012.6617871
ACTGTCG14700.012.5685428
ATAGACG20700.012.4972924
GTACCGT23700.012.2776666
TATGCCG369700.012.16780992-93
Produced by FastQC (version 0.11.5)