FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649141
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG19200.021.1796345
GTCTAGG51650.017.3306941
TATACCG52300.015.8156985
CCTAGAC53050.015.1577053
TAGAGCG56800.014.8881175
GCCGTCT347900.014.82197294-95
TGCCGTC427650.014.56997294-95
CTAGACA69650.014.5309044
GACAGTG35900.013.9009377
GTACCGT32550.013.769726
GTCTATG57650.013.7196071
GACGGTC22900.013.5192977
ACTGTCC35550.013.5180638
GTGTCCC30300.013.1152499
CCGTCTT299400.013.07157394-95
CGTCTTC266000.013.04531494-95
GGACTGC39550.012.9682356
GTCTTCT252800.012.87656994-95
GTGTATG78600.012.7743211
ATGCCGT473650.012.75987594-95
Produced by FastQC (version 0.11.5)