FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l03_n01_EXW15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l03_n01_EXW15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24924122
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA259000.018.16731594-95
TGCTTGA334550.017.3857894-95
GTCTAGG84500.017.1925961
CCTAGAC80550.016.8646893
TATACCG73750.016.6641795
CTAGACA110700.016.6541254
GTCTATG97550.015.6202651
TAGACGG39200.015.440085
TAGAGCG90550.015.3582715
CTAAGGT106700.015.1565454
ACTGTCC72600.015.0818668
CTGCTTG422900.014.69312194-95
TCTGCTT535450.014.63741694-95
GTCCTAA93750.014.4362551
GTACCGT62500.014.4141296
GCCGTCT1356400.014.28466594-95
TAGAGGT95800.014.1800714
TTCTGCT606450.014.15022594-95
GTCTAGA88400.013.9181311
TGCCGTC1619100.013.89298494-95
Produced by FastQC (version 0.11.5)