FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13991693
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157630.11265970458328382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG3207.52334E-9101.990632
ACTTGCG4405.456968E-1298.92
GTTTAGC6500.092.0530852
GTGTTAC3601.7047569E-890.658342
CGCGTAT3702.0620064E-888.2081152
AGGTCAG5052.1827873E-1186.17032
GGGATAC6551.8189894E-1274.741232
GCGTGAT3651.9600193E-674.51372
ACGTATG6603.6379788E-1274.1752
CGGATTA5908.54925E-1173.7559362
GTGAATG6653.6379788E-1273.61732
CGACTTA3702.1250635E-673.506762
CTTATCG6051.05501385E-1071.9272842
GAACGCT5353.25781E-971.1710362
GGACCAA10000.070.71352
CCTGGCG8500.070.393532
GGAACGT4901.4450052E-766.6061252
ATGTCGC4204.510668E-664.755962
GGACCTA3401.2361082E-463.9941252
CGCTGTT6008.065399E-963.4608422
Produced by FastQC (version 0.11.5)