FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10326432
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN105720.10237805274851952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA25900.041.9500852
CGCCTTA17852.978959E-828.8325672
CGGATAG22853.9108272E-1027.5286882
CGTCTAT19456.852679E-826.4607352
CGTCTAG15609.768741E-625.6597522
CGTGTTA15609.768741E-625.6597522
CGTCTTA25651.4952093E-924.5236052
CGCTTAA14051.4159904E-424.4204652
CGGACAG17001.9011526E-523.5465952
CGCGCTA14902.1086473E-423.0273512
CGCTCAT27753.7161954E-922.6677652
CGTATAG23053.5367884E-722.328042
CGCTCTA32509.986252E-1021.114312
GGTGTAT29858.620191E-921.073052
CGCCTAA16353.951207E-420.985172
CGTTCAA24656.746286E-720.8787542
CGTATAT27558.54543E-820.7566572
CGTCTAA19301.9099389E-1020.7405262
GGTCTAT44850.020.40032
CCTTCAG33801.6279955E-920.3022212
Produced by FastQC (version 0.11.5)