FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14304967
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150540.10523617426031114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT41400.030.9181692
CGTCCTA36500.028.96992
CGTATAG33400.026.6599752
CGTCTAT24009.476935E-1025.5074882
CGTATAC31100.025.0526642
CGTACTA35550.025.047632
CGGACAG23101.36387825E-824.0920772
CGCATAG21552.4035762E-723.242432
CGCCTTA24553.3665856E-822.6691252
CGTACAA29604.3655746E-1022.5619072
CGCACTA24753.6689016E-822.4859392
CGCTCAG32756.548362E-1122.091152
CGGTTTA35759.094947E-1221.7940652
CGTATAA38551.8189894E-1221.6547472
CGTTCAA38252.3646862E-1120.3696162
CGTTCAG21907.1639006E-620.3297542
GGTCTAG30250.020.2373472
CGTTCTA44250.020.123012
CGTCTTA38852.910383E-1120.0550272
CGTATTG36402.6739144E-1019.8759672
Produced by FastQC (version 0.11.5)