FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18026514
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190420.10563329105117052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG25250.033.263642
CGTTCAG26450.031.7545152
CGTGTTA24900.031.4824542
CGCTTAA26900.031.2233052
GGCCTAG32450.024.1575722
CGTATAG42200.023.8836082
CGTGGTA28055.3732947E-921.9583512
CGGCCTA33307.4578566E-1121.859442
CGTCTTA53050.021.109822
CGCTATA33601.9681465E-919.9977842
GGTCTAG36700.019.8343142
CGTATAC38050.019.13062
CGGATAG44851.0913936E-1118.7270222
CGTATAT51700.018.4118862
CGTCTAG27402.2768818E-618.3921222
CGCTGTA27402.2768818E-618.3921222
GACCTTA30953.635996E-718.0916962
CGTCTAA31000.018.0625132
CGTTTAG40751.1114025E-917.8630522
AGTCTTA59950.017.7461572
Produced by FastQC (version 0.11.5)