FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15490716
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166250.10732234714005472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG34400.033.946192
CGTCTAG23550.032.2308582
CGTCTAT28900.030.3049012
CGCCTTA25551.8189894E-1229.7078972
CGTTATA33700.025.9884742
CGTGTAC26152.564775E-1024.5606822
AGTGTTA26103.8744474E-822.3706682
CGTATAT43750.021.353122
CGCATAA30207.767085E-921.2669492
CGTACAA30207.767085E-921.2669492
GCCCGTA13950.00344846720.9273992
CGTGGTA22455.8631067E-620.8062152
AGTGTAC28309.1173206E-820.6316052
CGTATAG36801.6189006E-1020.6262
CGTCTTA45850.020.3751142
CGTGTTA20105.899627E-520.3339352
CGTATCG17405.226989E-420.13362
CGTGATA26351.0487674E-619.942582
CGCCTAA23859.891039E-619.5848872
CGCATAG21459.722716E-519.0541762
Produced by FastQC (version 0.11.5)