FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12757435
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135130.10592254634258376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20600.039.7502482
CGCTGTA19956.184564E-1132.2499352
CGCATAG18601.029548E-931.4460472
CGCTATG14354.2848715E-628.5315362
CGAGTAT24802.5465852E-1128.3014432
CGTTATG23551.2767487E-824.8363692
CGTTATA33001.8189894E-1224.813792
CGTCCTA26251.5061232E-924.5099472
CGTACTA36300.024.1692772
CGTTCAG22402.1601045E-723.5003052
CGGATAG29902.6557245E-1023.4741062
CGCATAA27552.6338967E-923.3533972
GTCCCTA22802.5624286E-723.088022
CGAGTAG38700.022.6704042
GGTCCAG33855.0931703E-1122.462792
CGTATAA34506.730261E-1122.0395772
CGTGTTG29656.15546E-921.6993642
CGCCTAA19404.4292232E-521.1045132
CGTGTTA20055.7089586E-520.4203242
CGCTCAG28751.0567419E-720.3442252
Produced by FastQC (version 0.11.5)