FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13646155
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143300.1050112650779652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG18450.042.6483732
CGTCTAG22750.037.0578842
CGTTTAG31250.030.5752262
CGTTATA32300.026.1011432
CGCTATA25551.7480488E-924.1976152
GGTACTG43500.023.2570152
CGTATAA39550.022.7375812
CGGTATG28205.467882E-921.9237252
CGTATAG35851.9099389E-1020.3809762
CGTCTTA39953.8198777E-1119.696182
CGTTCAG23351.14445975E-519.2563462
CGTATAT39557.0940587E-1018.4742852
CGGACAG18309.482872E-418.427692
CGTTCAA36705.6170393E-918.377482
CGCTGTG21601.373532E-418.2144092
CGGATAA59650.017.902512
CGTATTA47152.1827873E-1117.8805272
CGTGTAC25652.569692E-517.5296572
CGGTTAG25802.70168E-517.4277382
CGCATAT30005.21296E-616.8613382
Produced by FastQC (version 0.11.5)