FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10186511
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN107460.10549244977009302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG24400.042.4851072
CGCTATA17305.5842975E-1033.289552
CGCTGTG21705.456968E-1231.8475172
CGGACAG14701.6473132E-731.3420012
CGTCCTA22509.094947E-1230.7151582
CGTGGTA16951.6812919E-830.579252
CGTTATA23251.2732926E-1129.7243462
CGCATAG14254.576201E-628.2902762
GGTCTAG20600.027.9567572
CGTATAG24106.675691E-1026.2863122
CGCTTTA30900.026.0929742
CGTTCAG16401.3626215E-524.5814912
CGTACTA28151.5097612E-1024.5503032
AGTCTAG26201.7607817E-924.1793962
CGTCTAG16701.5680245E-524.139912
CCCGTTA11950.001519515224.096622
CGTACAG19802.220293E-623.269062
CGCTTAA15202.3003493E-422.733262
GGCCTAA30504.129106E-1022.6587242
CGTATAA28003.79805E-922.6250042
Produced by FastQC (version 0.11.5)