FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_POK15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_POK15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19011322
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN203530.10705725777513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAG31250.033.9545022
CGGTATG34950.028.7620012
CGTATAT47250.028.3663332
CGGATAG37650.028.1826882
CGTCCTA53950.027.948992
CGTCTTA40850.025.9749852
CGTTTAG38900.025.841442
CGTATAA39500.025.4489122
CGTATAG37900.025.0497572
GACCCTA60950.024.7390962
CGCTATA28000.023.9340952
CGTCTAG30502.3646862E-1123.8033082
CGATAAG39350.022.7074832
CGTTATG32706.002665E-1122.2018622
CGCTCTA55900.021.9788342
CGCTATG23204.7345384E-721.6644822
CGCATAG26005.9604645E-821.4793152
CGGTTTA44650.021.2628382
CGTGTAG57850.021.2379742
CGCCTTA32451.3187673E-920.6519172
Produced by FastQC (version 0.11.5)