FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_NON60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_NON60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15970404
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166110.10401114461475114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA27750.030.4455472
GCCCTAA40200.028.022022
CGTCTAT26851.10958354E-1025.1728532
GCCTCTA54650.023.704632
CGCATAG23004.0117084E-722.0399272
CGCCTAA26205.9046215E-821.4978082
CGGGTTG55900.021.1593822
CGTTCTA53350.021.1152
CCCGTTA19105.254377E-520.6423992
CGTCCTA41051.8189894E-1220.5813372
CGTATAA36102.0372681E-1020.2829762
CGAGTAG44450.020.2741992
CGCTTAG39403.092282E-1120.0136972
CGACTTA25351.0219264E-619.996782
CGGATAG33852.0045263E-919.9672432
CGCTCAG37203.0377123E-1019.683212
CGTGGTA26401.5078149E-619.2014522
CGCATTG33003.254172E-818.7747522
CGTCTAG24201.5296622E-518.6195892
CGCCTTA27402.1519572E-618.500672
Produced by FastQC (version 0.11.5)