FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_NON180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_NON180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10055102
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN104720.10414613397258428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG15300.059.5998572
CGCTATA18651.8189894E-1236.670692
CGCCATA21950.033.754022
GCCTCTA31900.030.3721052
CGTTCAG15302.5634836E-729.7999292
CGCCTTA17853.077912E-828.7356452
CGAGTAG26201.8189894E-1228.2786562
CGTGGTA16705.51423E-727.3017312
CGTACTA25856.002665E-1126.4568042
CGCTCAG21757.214112E-926.2033842
CGCTTTA26207.094059E-1126.1033742
GGTGTAT31000.025.7384852
CGGATAG24408.712959E-1025.693282
CGGATTA23001.3076715E-824.779292
GACCTTA16252.9261282E-624.5505582
GGTCTAG21250.024.1379412
CGTCCTA24702.788147E-823.0738322
CGTTCAA27453.4069672E-922.838472
CGTATTA27753.8635335E-922.5915682
CGTATAT28054.3728505E-922.3499472
Produced by FastQC (version 0.11.5)