FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n02_NON15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n02_NON15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20085445
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN212370.10573328099028924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG30650.032.4136662
CGGATAG44350.027.3788472
CGTTATA40550.027.2223282
CGTGTTA29300.026.372212
CGTATAG44550.026.0170292
CGTTCAG31101.8189894E-1224.8458422
CGGGCTA31801.8189894E-1224.2989232
CGTCTAT34500.023.9970722
CGTTTAG39800.023.5750142
CGTATAT55650.021.819442
CGCTCTA51400.021.475982
GGTCCTA61400.020.6750052
CGACTAG34851.6734703E-1020.5885942
CGTACAA40251.8189894E-1220.568922
CGCATAG26909.6701115E-820.5179442
CGTCTTG54750.020.1619262
CGTGATA33302.1063897E-919.8894672
CGAGTAG44600.019.8002762
CGTTGTA58850.019.6951352
GGGATTA70300.019.6277622
Produced by FastQC (version 0.11.5)