FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13991693
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGG7500.017.39978426-27
AACGACT10300.016.89400524-25
CTAGTCA9100.016.25351724-25
CCGAATG9550.015.790380526-27
CGCATAG7050.015.63180922-23
TCGACAG9650.015.62768524-25
GCGCTAA7850.015.51665924-25
ACGAATG9350.015.5078326-27
TACCGTT8800.015.48844526-27
AACGTAA11650.015.43420924-25
ATACAGC9050.015.38194524-25
ACGATTG9300.015.27938226-27
TACTGGC9500.015.26296826-27
CGACAAT10100.015.21763226-27
TAGTCGT8250.015.114964526-27
GAGTTAA11900.015.10905726-27
GCGACAA14400.015.10488224-25
TTACGTT8450.015.10130724-25
CAGTCAA17300.015.08651926-27
TCGTGGT10200.015.06844126-27
Produced by FastQC (version 0.11.5)